21878
Sigma
5(6)-Carboxyfluorescein N-hydroxysuccinimide ester
BioChemika, for fluorescence, mixture of isomers, ≥90% (HPLC)
| Synonym: | 5(6)-FAM SE, FLUOS |
| CAS Number: | 117548-22-8 |
| Linear Formula: | C25H15NO9 |
| Molecular Weight: | 473.39 |
| MDL number: | MFCD04119713 |
| PubChem Substance ID: | 24853087 |
Description
| Application | Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA) |
| Fluorescent label for primary and secondary amine groups on proteins and other biomolecules. |
Properties
| grade | for fluorescence |
| product line | BioChemika |
| assay | ≥90% (HPLC) |
| fluorescence | λex 492 nm; λem 517 nm in DMF |
| solubility | DMF: soluble |
| DMSO: soluble | |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
References
| reference | G.P.A. Vigers et al. J. Cell Biol. 107, 1011, (1988) Abstract |
| M. Lu-Steffes et al. Clin. Chem. 28, 2278, (1982) Abstract | |
| Banks, P.R., Paquette, D.M., Comparison of three common amine reactive fluorescent probes used for conjugation to biomolecules by capillary zone electrophoresis. Bioconjug. Chem. 6, 447, (1995) |
